| Abstract
| - The treatment of hairy roots of Hyoscyamus albus with copper sulfate (Cu2+) and methyl jasmonate (JAMe)produced several phytoalexins having the vetispyrane skeleton. Lubimin and solavetivone were isolatedafter treatment with Cu2+. Seven sesquiterpenoid phytoalexins were isolated from the culture mediumafter treatment with JAMe, including lubimin, solavetivone, 3-hydroxysolavetivone and four newcompounds (1−4). Structures of the new compounds were elucidated to be (3R,4S,5R,7S,9R)-3-hydroxy-9-tigloyloxysolavetivone (1), (3R,4S,5R,7S,9R)-3-hydroxy-9-(3-methylbutenoyloxy)-solavetivone (2),(3R,4S,5R,7S,9R)-3-hydroxy-9-isobutanoyloxysolavetivone (3); and (3R,4S,5R,7S,9R)-3,9-dihydroxysolavetivone (4). The induction pattern of phytoalexins in hairy roots treated with JAMe was different inthose treated with Cu2+, and co-treatment with JAMe and Cu2+ gave only solavetivone.
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