A new lupane-type triterpene saponin, pulsatilloside C(1), was isolated from the roots ofPulsatilla chinensis. Its structure was established tobe 3β,23-dihydroxylup-20(29)-en-28-oicacid28-O-α-l-rhamnopyranosyl-(1→4)-β-d-glucopyranosyl-(1→6)-β-d-glucopyranoside.