| Abstract
| - Microbial transformation studies of the antifungal alkaloid sampangine (2) have revealed that it ismetabolized by a number of microorganisms. Using a standard two-stage fermentation technique,Beauvaria bassiana (ATCC 7159), Doratomyces microsporus (ATCC 16225), and Filobasidiella neoformans(ATCC 10226) produced the 4‘-O-methyl-β-glucopyranose conjugate (3), while Absidia glauca (ATCC22752), Cunninghamella elegans (ATCC 9245), Cunninghamella species (NRRL 5695), and Rhizopusarrhizus (ATCC 11145) produced the β-glucopyranose conjugate (4). Metabolites 3 and 4 have beencharacterized on the basis of spectral data. Both 3 and 4 had significant in vitro activity againstCryptococcus neoformans but were inactive against Candida albicans. Metabolite 4 was inactive in vivoin a mouse model of cryptococcosis.
|
