| Abstract
| - Based on a brief revision of what constitutes state-of-the-art “quantitative experimental conditions” for1H quantitative NMR (qHNMR), this comprehensive review contains almost 200 references and coversthe literature since 1982 with emphasis on natural products. It provides an overview of the backgroundand applications of qHNMR in natural products research, new methods such as decoupling andhyphenation, and analytical potential and limitations, and compiles information on reference materialsused for and studied by qHNMR. The dual status of natural products, being single chemical entities andvaluable biologically active agents that need to be purified from complex matrixes, results in an increasedanalytical demand when testing their deviation from the singleton composition ideal. The outcome andversatility of reported applications lead to the conclusion that qHNMR is currently the principal analyticalmethod to meet this demand. Considering both 1D and 2D 1H NMR experiments, qHNMR has proved tobe highly suitable for the simultaneous selective recognition and quantitative determination of metabolitesin complex biological matrixes. This is manifested by the prior publication of over 80 reports on applicationsinvolving the quantitation of single natural products in plant extracts, dietary materials, and materialsrepresenting different metabolic stages of (micro)organisms. In summary, qHNMR has great potentialas an analytical tool in both the discovery of new bioactive natural products and the field of metabolomeanalysis.
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