| Abstract
| - Tobacco mosaic virus (TMV) infection of tobacco is a well-known and extensively studied model system for which anumber of genes and proteins involved in the systemic acquired resistance (SAR) have been characterized. Little isknown about the metabolic changes connected with the infection and SAR. Here we describe the use of NMR spectroscopyin combination with multivariate data analysis to study the metabolic changes. Particularly 2-D NMR methods, such as2-D J-resolved spectra and their projected spectra, are shown to be powerful tools in the metabolomic studies. Themacroscopic view of the metabolomes obtained by NMR spectroscopy of crude extracts enabled the identification of aseries of totally different metabolites that seem connected with resistance, such as the clearly increased 5-caffeoylquinicacid, α-linolenic acid analogues, and sesqui- and diterpenoids in the infected plant parts.
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