The selective C-terminal deprotection of O-glycopeptide(methoxyethoxy)ethyl esters is achievedunder mild conditions (pH 6.6, 37 °C) by enzymatic hydrolysis usingpapain or lipase M from Mucorjavanicus to give building blocks useful for chain-extendingglycopeptide synthesis. On the otherhand, the selective removal of acetyl protecting groups from thesaccharide portion of glycopeptidesis accomplished by alternative enzymatic hydrolysis with lipase WG fromwheat germ to furnishmodel substrates for enzymatic glycosyl transfer reactions in order toextend the carbohydrateside chain of these conjugates.