| Abstract
| - Synthetic methodology that allows for incorporation of isotopic carbon at the C-3 and C-4 positionsof bile salts is reported. Three [3,4-13C2]-enriched bile salts were synthesized from either deoxycholicor lithocholic acid. The steroid 3α-OH group was oxidized and the A-ring was converted into theΔ4-3-ketone. The C-24 carboxylic acid was next converted into the carbonate group and selectivelyreduced to the alcohol in the presence of the A-ring enone. Following protection of the 24-OH group,the Δ4-3-ketone was converted into the A-ring enol lactone. Condensation of the enol lactone with[1,2-13C2]-enriched acetyl chloride and subsequent Robinson annulation afforded a [3,4-13C2]-enrichedΔ4-3-ketone that was subsequently converted back into a 3α-hydroxy-5β-reduced bile steroid. C-7hydroxylation, when necessary, was achieved via conversion of the Δ4-3-ketone into the corresponding Δ4,6-dien-3-one, epoxidation of the Δ6-double bond, and hydrogenolysis/hydrogenation ofthe 5,6-epoxy enone system. The [3,4-13C2]-enriched bile salts were subsequently complexed tohuman ileal bile acid binding protein (I-BABP), and 1H-13C HSQC spectra were recorded to showthe utility of the compounds for investigating the interactions of bile acids with I-BABP.
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