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| - Universal Solid Supports for the Synthesis of Oligonucleotides viaa Transesterification of H-phosphonate Diester Linkage
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| Abstract
| - Three universal solid supports exhibiting an hydroxyl function were prepared. The introduction ofa first H-phosphonate diester linkage which was kept throughout the elongation allowed the releaseof 3‘-hydroxyl oligonucleotides by a transesterification mechanism. The transesterification wasperformed in a few minutes with either amino alcohols or K2CO3/methanol. Starting from a hydroxylsolid support, tandem oligonucleotides were synthesized and the solid support was easily recyclable.This strategy was extended to the release of an oligonucleotide from the solid support by a nonbasictreatment opening the way to the synthesis of base-sensitive oligonucleotides thanks to the selectivedeprotection of a hydroxyl in β of the H-phosphonate diester linkage.
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