Abstract
| - We synthesized a three-way branched oligodeoxynucleotide (ODN) 30-mer using a new branch unitwith acid-labile DMTr and oxidatively cleavable TrS groups as orthogonal protecting groups. The branchedODN was successfully synthesized using 5-[3,5-bis(trifluoromethyl)phenyl]-1H-tetrazole and (2R,8aS)-(+)-(camphorylsulfonyl)oxaziridine as the activator of phosphoramidite units and the oxidizing reagent,respectively. We also found that the TrS group was orthogonal to the Lev, TBDMS, and Fmoc groups.These results indicate the possibility of the synthesis of more complex four- and five-way branchedODNs by the combined use of DMTr, TrS, Lev, TBDMS, and Fmoc groups.
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