About: Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules byTwo-Photon Excitation

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Attributs	Valeurs
type	work Article
Is Part Of	http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/w
Subject	Letter
Title	Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules byTwo-Photon Excitation
has manifestation of work	http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/101021jp971915b/m/web http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/101021jp971915b/m/print
related by	http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/101021jp971915b/authorship/2 http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/101021jp971915b/authorship/3 http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/101021jp971915b/authorship/4 http://hub.abes.fr/acs/periodical/jpcafh/1997/volume_101/issue_38/101021jp971915b/authorship/1
Author	Xie X. Sunney Sánchez Erik J. Holtom Gary R. Novotny Lukas
Abstract	We report fluorescence imaging of single dye molecules on a glasssubstrate by two-photon excitation withfemtosecond pulses from a mode-locked Ti:sapphire laser. Thesingle-molecule images exhibit a high signalto background ratio (>30:1) and a high spatial resolution (fwhm <λ/3). A quadratic intensity dependenceofsingle-molecule emission rate is experimentally verified, andsingle-molecule emission spectra are recordedwith two-photon excitation. A comparison of photobleaching ratesis made between one-photon and two-photon excitation schemes.
article type	rapid-communication
is part of this journal	The Journal of Physical Chemistry A

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