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| - Detection of Bilayer Packing Stress and Its Release in Lamellar-Cubic Phase Transition byTime-Resolved Fluorescence Anisotropy
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| - An introduction of nonlamellar-forming lipids into planar bilayers generates packing stress, which is importantfor the biological functions of plasma membranes and is a driving force for the lamellar-nonlamellar phasetransition. We have investigated the phase behavior of a binary system consisting of egg yolk phosphatidylcholine and monoolein (MO) and the changes in the local orientation order of lipids in a lamellar-bicontinuouscubic phase transition. Small-angle X-ray scattering has revealed that the lamellar-bicontinuous cubic phasetransition occurs at an MO molar fraction (XMO) between 0.6 and 0.7. These phases were dispersed to formliposomes and cubosomes to monitor the anisotropy of the incorporated fluorescence probe, in which PluronicF127, used as a dispersion stabilizer of the cubic phase, has been proven not to alter the cubic structure andthe location of the probes. Time-resolved fluorescence anisotropy measurements on these dispersions haverevealed that the order parameter of the probe in the lamellar phase increases with increasing XMO, and thatit decreases during the transition to the cubic phase. This observation suggests that packing stress generatedby the addition of the nonlamellar-forming lipid is released by the phase transition.
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