Single-molecule electrical conduction studies are used to evaluate how the molecular linking unit influencesthe tunneling efficiency in metal−molecule−metal (m-M-m) junctions. This work uses conducting-probeatomic force microscopy (CP-AFM) to compare the molecular conduction of two π-bonded molecules: onewith a single thiol linker, and another with a conjugated double thiol linker at both ends of the molecules.The results demonstrate that the molecule with conjugated double thiol linkers displays higher conduction ingold−molecule−gold junctions than nonconjugated single thiol−gold contacts.