| Abstract
| - The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion withinanisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionicfluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystalwere subsequently analyzed. All data could be described by a classical pore diffusion model. There was nochange in the adsorption characteristics, but diffusion decreased with the introduction of a second solute inthe solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions,while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that theoutcome of binary transport depends on the solute, protein, and crystal characteristics.
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