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| - RNA Selectively Interacts with Vesicles Depending on Their Size
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| - RNA and vesicles are two important molecular classes in the origin of life and early evolution, but they arenot generally considered as interacting partners. The present paper reports about the interaction betweentRNA (Esherichia coli) and vesicles made of the zwitterionic surfactant POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), partially positively charged with small molar fractions (max 10%) of the single-chained CTAB (cetyltrimethylammonium bromide). CTAB is capable to insert efficiently in POPC vesicles(as determined by zeta-potential measurements), and the binding of tRNA to such charged vesicles operatesa strong selection being critically dependent upon the vesicle size. The binding of tRNA to the vesicles issize-selective as it induces a strongly pronounced process of aggregation of large vesicles (ca. 160-nm diameter)but not of small ones (ca. 80-nm diameter) that are stable against vesicle aggregation (as followed by dynamiclight-scattering and optical density measurements). The aggregation of the large vesicles is fully reversibleupon the addition of RNase A. The selective behavior of tRNA with respect to differently sized vesicles isobservable in separated samples as well as in a mixture of both populations. In the latter case, the fraction oflarge vesicles readily aggregates in the presence of the small ones that remain unaltered in the mixture. Thiskind of discrimination capability of RNA might have been of importance in the early phases of the formationof the protocells.
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