Abstract
| - DNA was studied in lipid reverse micelles with the aim of investigating the interactions of DNA with lipidsin a restricted compartment with minimal water content. Circular dichroic (CD) spectra of DNA at low watercontent showed the characteristic polymer-salt-induced (psi) spectra of condensed DNA. Dynamic lightscattering showed a peak around a radius of 400 nm (corresponding to DNA-containing micelles), and a peakaround 2.5 nm (corresponding to “empty” micelles). Fourier Transform-IR (FT-IR) spectroscopy was carriedout and analyzed in terms of three distinct states of water inside the micelle water pool, where the localconcentration of DNA reached an estimated value of ca. 600 mg/mL, comparable to that found in restrictedbiological compartments.
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