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| - Plasmid DNA Encapsulation within Cationic Diblock Copolymer Vesicles for Gene Delivery
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| - We report the design and structural characterization of cationic diblock copolymer vesicles loaded with plasmidDNA based on a single emulsion technique. For this purpose, a DNA solution was emulsified in an organicsolvent and stabilized by an amphiphilic diblock copolymer. The neutral block forms an interfacial brush,whereas the cationic attachment complexes with DNA. A subsequent change of the quality of the organicsolvent results in the collapse of the brush and the formation of a capsule. The capsules are subsequentlydispersed in aqueous medium to form vesicles and stabilized with an osmotic agent in the external phase.Inside the vesicles, the plasmid is compacted in a liquid−crystalline fashion as shown by the appearance ofbirefringent textures under crossed polarizers and the increase in fluorescence intensity of labeled DNA. Thecompaction efficiency and the size distribution of the vesicles were determined by light and electron microscopy,and the integrity of the DNA after encapsulation and subsequent release was confirmed by gel electrophoresis.We demonstrate reverse transfection of in vitro cultured HeLa cancer cells growing on plasmid−copolymervesicles deposited on a glass substrate.
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