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| - Enhanced Förster Resonance Energy Transfer (FRET) on a Single Metal Particle
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| - We examined the effect of a metallic silver particle on Förster resonance energy transfer (FRET) between anearby donor−acceptor pair. A donor-labeled oligonucleotide was chemically bound to a single silver particle,and then an acceptor-labeled complementary oligonucleotide was conjugated by hybridization. Thephotophysical behavior of FRET between the donor−acceptor pair on the metal particle was investigatedusing both ensemble emission spectra and single-molecule fluorescence detections. Both the emission intensitiesand lifetimes indicated an enhanced FRET efficiency due to the metal particle. This interaction led to anincrease in the Förster distance for energy transfer from 8.3 to 13 nm. The rate constant of FRET near thesilver particle was 21 times faster than that of unbound donor−acceptor pairs. These results suggest the useof metal-enhanced FRET for measuring proximity of large biomolecules or for energy-transfer-based assays.
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