| Abstract
| - Fibrin bilayers are formed in a stepwise manner on four model surfaces: hydrophobic, neutral hydrophilic,positively charged, and negatively charged. These surfaces are self-assembled monolayers (SAMs) ofalkanethiolates on gold with different terminal groups. A surface plasmon resonance (SPR) sensor is used tomonitor the formation of fibrin bilayers in real time. The structures of the fibrin bilayers formed on SAMsof different surface chemistries are proposed, based on the amount of fibrinogen in each layer. The efficiencyof the physically adsorbed fibrinogen to promote fibrin formation is assessed by the ratio of the amount offibrinogen in the second layer to that in the first layer. It is shown for the first time that the orientation/conformation of an adsorbed fibrinogen layer determined by underlying surface chemistry affects its reactivitywith soluble fibrinogen, resulting in fibrin bilayers with different molecular arrangements. Bovine aorticendothelial cells cultured on fibrin bilayers formed on CH3− and OH− SAMs exhibited different morphologiesand growth characteristics. Results show that fibrin bilayers with different molecular arrangements and thusdifferent mechanochemical properties lead to different cellular responses.
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