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| - Spatial Confinement Induced Enzyme Stability for Bioelectronic Applications
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| - The stability of glucose oxidase (GOx) immobilized on a silicon electrode can be enhanced using spatialconfinement as described in this paper. We show that when GOx is assembled in semiopen spatially confinedstructures created on the unmodified surface of a silicon electrode, the enzyme's activity is preserved underdestabilizing conditions. GOx-immobilized silicon electrodes were treated with guanidinium chloride andhigh temperature. Cyclic voltammetry measurement of the treated electrodes showed the proper redoxcharacteristics of GOx and indicated the GOx-catalyzed electrooxidation of glucose. When GOx wasimmobilized on flat silicon electrodes, voltammetric measurement showed null result presumably caused bydenaturation of the enzyme. The effect of spatial confinement on enzyme stability is also revealed by analyzingthe characteristic rate constants and the kinetic parameter for the complete catalytic process of glucose.
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