| Abstract
| - A much-improved Poisson−Boltzmann equation for two globular proteins using bispherical coordinates isused to establish the potential of mean force (PMF) between two globular lysozyme particles. Calculationspresented here include previously ignored ion−protein nonelectrostatic potentials. The lysozyme−lysozymePMF is used to calculate osmotic second virial coeffiencients. The theoretical PMF curve as a function ofsodium chloride concentration is successfully compared with that from experiment. Our theoretical PMFshows how the second virial coefficient and the phase diagram depends on salt concentration, pH and, mostnotably, on the choice of salt in the aquous solution.
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