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| - Hybridization of ssDNA with a Complementary DNA Probe Tethered to a GoldNanoparticleEffect of Steric Hindrance Caused by Conformation
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| - We investigated hybridization of probe DNA I and II tethered to gold nanoparticles (bulky probe I and II,respectively) with target single-strand DNA (ssDNA), where DNA I and II have the sequences complementaryto the 50mer sequence starting from the 3501st (center) nucleotide and the 7199th (terminus) one of thetarget ssDNA (7249 bases), respectively. The hybridization rate of the bulky probe I was found to be slowerthan that of the bulky probe II. This finding is explained in terms of steric hindrance of the bulky probes. Thesteric hindrance is more significant when the probe hybridizes with a target ssDNA at a site nearer to itscenter. The freely jointed chain model is employed to analyze the hybridization kinetics semiquantitatively.The analysis shows that the ratio of the rate constant at the center to that at the terminus is magnified morewhen a bulkier probe is used.
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