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| Title
| - Evaluation of Hydrodynamic Size and Zeta-Potential of Surface-Modified Au Nanoparticle-DNA Conjugates via Ferguson Analysis
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| Abstract
| - We evaluate Ferguson analysis as a method for simultaneously measuring the size and zeta-potential of gold nanoparticle-DNA (Au NP-DNA) conjugates. This approach is highly suitable when the particle size is <20 nm and biomolecular conformation results in a nonspherical or nonlinear conjugate, causing conventional dynamic light scattering and zeta-potential measurements to be unable to achieve consistent results. We show the applicability of the method by varying the size of gold nanoparticles (5-20 nm) and the loading of methoxypolyethylene glycol/DNA on particle surface and buffer concentrations. Due to the low ionic mobility of tris-borate-EDTA buffer (TBE) and relatively high zeta-potential of Au NP-DNA, Henry’s solutions with higher order correction terms are necessary for obtaining the zeta-potential from the measured free mobility and size.
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| - Surface-Modified Au Nanoparticle-DNA Conjugates
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