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À propos de : Insertion of Luciferase in Langmuir−Blodgett FilmsThrough Enzyme−Glycolipidic Vesicles        

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  • Insertion of Luciferase in Langmuir−Blodgett FilmsThrough Enzyme−Glycolipidic Vesicles
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  • A proteoglycolipidic interfacial film is obtained from the spreading of two types of glycolipid vesiclesassociated with luciferase through adsorption or inclusion processes. The vesicles' disintegration is followedby recording the increase in surface pressure versus time. The interfacial film thus obtained is compressedand transferred onto substrates, and the luciferase activity is assayed through bioluminescence studies.It was shown that the luciferase is inserted in an active form in the transferred films. Its kinetic behaviordepends on the surface pressure at which the interfacial film was transferred and on the type of enzymeassociation.
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