Spatially rhythmic (i.e., concentric ring and symmetric radial band) patterns appeared from dropletsof collagen solutions during evaporation. A morphology diagram representing the pattern generation andbifurcation versus collagen concentration and evaporation time was determined. The pattern structureswere examined using an atomic force microscope and polarizing optical microscope. The characteristicwidth of the radial bands (40−200 μm) showed stepwise increases toward the pattern center because ofa systematic generation of fusing and dislocation. The spatiotemporal evolution of the patterns involvedan oscillatory motion toward the droplet center (for the ring patterns) and a periodic segmentation alongthe droplet edge (for the radial patterns), suggesting the onset of distinct interfacial instabilities coupledwith evaporation.