| Abstract
| - Adhesive templates for biomolecule patterning were fabricated on silicon and gold by low-voltage (1 kV)electron beam lithography of an inert self-assembled monolayer, followed by backfilling the exposed regionswith an amine-terminated monolayer. Amine-terminated monolayers selectively attached either the desiredmaterials or linker molecules that subsequently bound other materials including antibodies. Lines (300nm wide) of 20-nm polystyrene beads were formed on gold by exposing a mercaptohexadecanoic acid(MHDA) monolayer, then backfilling with cysteamine, and selectively attaching aldehyde-coated beadsto the amines. Attachment density was found to vary sharply around a critical dose, making the techniqueuseful for patterns such as gradients which require varying density. An optimal dose of 200 μC/cm2 wasfound for attaching fluorescent polystyrene spheres to MHDA−cysteamine templates. A cycling processwas developed for aligning patterns of two or more kinds of polystyrene spheres. Biotin was tethered tothe amine templates, making the technique applicable to high-resolution patterning of biomaterials withthe widely used avidin−biotin binding system.
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