| Abstract
| - Streptavidin was crystallized on giant bilayer vesicles (20−60 μm) in sucrose solution at various pHvalues. The streptavidin-coated vesicles exhibited unique roughened spherical and prolate ellipsoidalshapes, illustrating resistance to curvature of the two-dimensional crystals. Studies indicated that thespheroids and prolate ellipsoids correspond to different crystal morphologies. Through confocal microscopy,the various crystal morphologies on vesicle surfaces were observed under different solution conditions.Unlike two-dimensional (2D) streptavidin crystals grown in ionic buffer that assume the P1, P2, and C222lattices at pH 4, 5.5, and 7, respectively (Wang et al. Langmuir1999, 15, 1541), crystals grown in sucrosewith no added salt show only the lowest density C222 lattice due to strong electrostatic interactions.
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