| Abstract
| - Organized multilayers of nanoparticles (9-, 20-, and 45-nm-diameter silica or 12-nm magnetite) andglucose oxidase (GOx) were assembled in alternation with oppositely charged polyelectrolytes on 420-nmlatex particles. Stepwise growth of the multilayer films on latex was confirmed by microelectrophoresisand transmission electron microscopy. The inclusion of silica layers on latex yields a higher surface area,resulting in greater GOx adsorption and thereby increasing the catalytic activity of the bioreactor. Thebioactivity was proportional to the core surface area and also to the number of GOx layers in the shells.Also, the presence of magnetic nanoparticles allows self-stirring of the nanoreactors with a rotating magneticfield and enhances its productivity. The ensemble of GOx and fluorescent dyes in the shells demonstratedthe correlation between Ru-bpy fluorescence and glucose concentration in solution.
|
