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| - Direct Infrared Spectroscopic Evidence of pH- and IonicStrength-Induced Changes in Distance of AttachedPseudomonas aeruginosa from ZnSe Surfaces
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| - Control of bacterial adhesion is important in many industrial and medical applications. Ionic strengthand pH are known to affect the process of attachment; however their influence on bacteria/surface distanceshas been inferred but not quantitatively established. The distances between attached bacteria and surfaceshave only recently been measured. In this paper ATR-IR spectroscopy has been used to study the influenceof pH and ionic strength on the distance of freshly attached Pseudomonas aeruginosa from ZnSe surfaces.Reversible changes in the absorbance of P. aeruginosa were observed for changes of pH between 4 and10 at a constant ionic strength of 0.003 mol L-1 and for changes of ionic strength between 0 and 0.15 molL-1 at a constant pH of 6.3. An increase in ionic strength from 0 to 0.003 mol L-1 led to an increase inamide II absorbance of 30%, which corresponds to an average movement of 120 nm toward the surface.A movement of this magnitude is likely to be due to changes in the length of bacterial surface polymers.The influence on bacterial surface polymer lengths of ionic strengths in this range has not been widelyconsidered in models of bacterial attachment.
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