Microarrays of lipids containing gangliosides were fabricated on surfaces coated with γ-aminopropylsilane.A fluorescence microarray scanner was used to detect the binding of toxins to these arrays. The specificbinding of cholera and tetanus toxins to microspots containing GM1 and GT1b gangliosides, respectively,was demonstrated. These results suggest the possibility of using ganglioside microarrays for multiplexedtoxin detection and the screening of compounds that can inhibit toxin binding.