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| - Effect of Ionic Strength on Two-Dimensional StreptavidinCrystallization
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| Abstract
| - We study two-dimensional (2D) crystallization of streptavidin under low ionic strength conditions. Weinvestigate crystals on both giant unilamellar lipid bilayer vesicles and lipid monolayers at the air−waterinterface. In contrast to 2D crystallization in high ionic strength buffers, the electrostatic repulsions underthese conditions favor the low density C222 crystal form. The crystals grown on giant vesicles and thoseon lipid monolayers show the same crystal structure and similar crystallization trends despite the differencein surface geometries. When the ionic strength of the crystallization solution is progressively increasedat pH 4, we observe a transition from C222 to the denser P1 space group commonly seen in high ionicstrength conditions at this pH. A new crystal structure appears at intermediate ionic strength, illustratingthe role of electrostatic repulsions in these ordering processes.
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