| Abstract
| - A glycocalyx-mimetic film was created by the assembly of biotin chain-terminated glycopolymers ontoa polymeric lipid membrane electrostatically coupled to a polyelectrolyte multilayer. Varying molarcompositions (10, 25, and 50 mol %) of acrylate-derivatized biotin−phosphoethanolamine/phosphorylcholinelipid mixtures were prepared as unilamellar vesicles, fused onto the alkyl chains of an amphiphilic terpolymer,and photopolymerized in situ as a planar assembly. Polarized external reflection infrared spectroscopyconfirmed the presence of streptavidin. Notably, IR spectroscopy revealed an increase in the conformationaland orientational disorder of the lipid hydrocarbon chains with increasing mole fraction of biotinylatedlipid. Correlative images obtained by confocal fluorescence microscopy demonstrated that biotinylatedlipids cluster at high surface density. Despite the presence of biotin microdomains, the size and hydrophiliccharacteristics of coupled glycopolymer chains produced a uniform carbohydrate surface coating.
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