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| - Phospholipid Monolayers Supported on Spun CastPolystyrene Films
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| - We investigated the use of spun cast polystyrene (PS) films as a hydrophobic solid support for phospholipidmonolayers. Fluorescent microscopy indicated that a 1-palmitoyl-2-oleoyl-SN-glycero-3-phosphocholine(POPC) monolayer containing fluorescent lipid formed on ∼80 nm thick films of 2450 Mr PS exposed tophospholipid vesicle solutions. Qualitative fluorescence photobleaching experiments suggested that thephospholipid layer was continuous and the lipid diffusion rate was similar to those observed in glass-supported POPC bilayers. Examination of lipid diffusion by real-time, single-molecule fluorescence detectionand fluorescence correlation spectroscopy (FCS) indicated that the lipid diffusion contained multiplecomponents. We interpreted this as being caused by the presence of surface heterogeneities that confinelipid diffusion. In situ atomic force microscopy revealed the presence of 5−20 nm outgrowths on the PSfilm resulting from surface rearrangement upon exposure of the film to aqueous conditions. It is possiblethat such rearrangements play a role in determining the lipid translational dynamics. Our studies indicatethat PS films can be suitable supports for phospholipid monolayers, but that immersion into an aqueousenvironment can significantly influence the film topography and the dynamics of the lipid in the supportedmonolayer.
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