| Abstract
| - A novel approach in the fabrication of microarrays of dye and protein on fused silica plates using thelaser-induced backside wet etching (LIBWE) technique is described. The surface of fused silica plates wasinitially precoated using trimethoxysilane self-assembled monolayers (SAMs) and then etched using theLIBWE method to obtain the desired microstructures on the plate surface. Using this technique, the SAMson the nonirradiated areas were able to survive the LIBWE process and were used as templates for thesubsequent deposition of dye molecules or proteins via chemical bonding or physical adsorption. In thecase of fused silica plates precoated with fluorinated SAMs, the LIBWE method is used to remove the SAMsto expose the etched silica surfaces, on which a thin layer of pyranine molecules can be site-selectivelydeposited using an aqueous solution of pyranine. In another application, an ethanol solution of rhodamine6G was preferentially deposited onto the nonirradiated areas. In yet another application, bovine serumalbumin was preferentially deposited onto the laser-irradiated areas; in this case, the fused silica plateswere precoated with poly(ethylene oxide) SAMs. Interestingly, when an aqueous suspension of polystyrene(PS) microbeads was cast onto the fused silica precoated with the fluorinated SAMs, hexagonally close-packed PS microbeads were deposited into the etched cavities. Depositions of the dye, protein, and microbeadswere confirmed by visualization using a fluorescence microscope and scanning electron microscope.
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