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À propos de : Micropatterning of Cell-Repellent Polymer on a Glass Substrate forthe Highly Resolved Virus Microarray        

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  • Micropatterning of Cell-Repellent Polymer on a Glass Substrate forthe Highly Resolved Virus Microarray
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  • The development of a simple and easily accessible method to control cellular behavior under a spatially controlledsurface is critical for fundamental studies in biotechnology. We fabricated a microarray of Spodoptera frugiperda 9(Sf9) cells on a glass surface by microcontact printing cell-repellent polymeric molecules of poly(ethylene glycol)-branched-poly(methyl methacrylate) as a template for cell micropatterning. The polymer micropatterns enabled thestable confinement of Sf9 cells on the surface, resulting in the formation of a cell microarray. Subsequently, thepatterned Sf9 cells were infected with recombinant baculovirus modified with green fluorescent protein (GFP) to forma virus microarray, and GFP expression in the virus microarray was verified with confocal fluorescence microscopy.
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