| Attributs | Valeurs |
|---|
| type
| |
| Is Part Of
| |
| Subject
| |
| Title
| - Spatially Controlled Cell Adhesion via Micropatterned SurfaceModification of Poly(dimethylsiloxane)
|
| has manifestation of work
| |
| related by
| |
| Author
| |
| Abstract
| - Spatial control of cell growth on surfaces can be achieved by the selective deposition of molecules that influencecell adhesion. The fabrication of such substrates often relies upon photolithography and requires complex surfacechemistry to anchor adhesive and inhibitory molecules. The production of simple, cost-effective substrates for cellpatterning would benefit numerous areas of bioanalytical research including tissue engineering and biosensor development.Poly(dimethylsiloxane) (PDMS) is routinely used as a biomedical implant material and as a substrate for microfluidicdevice fabrication; however, the low surface energy and hydrophobic nature of PDMS inhibits its bioactivity. Wepresent a method for the surface modification of PDMS to promote localized cell adhesion and proliferation. Thinmetal films are deposited onto PDMS through a physical mask in the presence of a gaseous plasma. This treatmentgenerates topographical and chemical modifications of the polymer surface. Removal of the deposited metal exposesroughened PDMS regions enriched with hydrophilic oxygen-containing species. The morphology and chemicalcomposition of the patterned substrates were assessed by optical and atomic force microscopies as well as X-rayphotoelectron spectroscopy. We observed a direct correlation between the surface modification of PDMS and themicropatterned adhesion of fibroblast cells. This simple protocol generates inexpensive, single-component substratescapable of directing cell attachment and growth.
|
| article type
| |
| is part of this journal
| |
