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Title
| - The Transmitting Tissue in Brugmansia suaveolens L.: Ultrastructure of the Stylar Transmitting Tissue
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Abstract
| - The stylar transmitting tissue of the angel's trumpet, Brugmansia (Datura) suaveolens, was studied at two developmental stages: about 6 d before anthesis and after anthesis. Histochemical localization of polysaccharides was carried out with PATAg and immunohistochemistry with gold-conjugated antibodies recognizing pectins. Before anthesis the transmitting tissue forms a central core of polyhedral meristematic, still dividing, cells with narrow intercellular spaces. Epitopes for unesterified pectins are present in the walls and the spaces between the cells, while methylesterified pectins are confined to the middle lamella and intercellular spaces. PATAg positive material and the antibody against unesterified pectin was found in plasmalemma invaginations and multivesicular bodies. Dictyosome cisternae and vesicles contained epitopes for both kinds of pectins. Plastids are poorly differentiated and lack starch. Nutrients are stored as lipid bodies, which are digested by small vacuoles. After anthesis the transmitting tract cells form cylindrical files separated by voluminous spaces filled with a mucous secretion reacting with PATAg and with the antibody against unesterified pectins. Dictyosome vesicles contain epitopes for the same kind of pectins. The cells are vacuolized and have leucoplasts. This study shows pectin synthesis by different parts of the endomembrane system and changes in pectin esterification during stylar development.Copyright 1993, 1999 Academic Press
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