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| - Tentoxin sensitivity of citrus: cotyledon-dependency of growth inhibition and reversibility of chlorosis
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| - Tentoxin is a cyclic tetrapeptide, produced by the fungus Alternaria alternata, that induces chiorosis in germinating seedlings of some angiosperms. Since the most pronounced chiorotic effect of tentoxin is at the initial stages of germination most studies have evaluated the effects of tentoxin on cotyledons. In this preliminary work a unique biological system was established for the study of the mechanism of tentoxin induced chiorosis in developing citrus seedlings. This system was used to compare the effects of tentoxin on the in vitro germination of intact versus decotyle donized embryos. It is demonstrated here that the chlorotic effect of tentoxin is reversible and that ten- toxin blocks the ability of decotyledonized embryos to utilize nutrients from the growth medium and, there fore, to compensate for the lack of cotyledons. The citrus system offers a unique way to study the relation between the effect of tentoxin on the activity of choloplast ATPase and the induction of chlorosis.
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