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| - In vivoadministration of antibody to murine IL-5 receptor inhibits eosinophilia of IL-5 transgenic mice
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| - We investigated the in vivo role of interleukin 5 (IL-5) and its receptor (IL-5R) in eosinophil growth and differentiation. When mice were administered IL-5 I.p., an increase In the number of eosinophlls was observed within 5 days in peripheral blood and the peritoneal cavity. Hypereoslnophilla was observed in IL-5 transgenic mice who displayedconstitutive production of IL-5. A binding assay with 35S-labeled IL-5 revealed the presence of two classes of IL-5 binding sites (low and high affinity) on the surface of eosinophils. IL-5Rs on eosinophils were recognized using mAbsagainst murine IL-5R. When the IL-5 transgenic mice were passively administered with anti-murine IL-5R mAbs, the number ofrecognizable eosinophils in peripheral blood dropped within 5 days to normal levels. The antibody treatment also preventedthe Increase In the number of eosinophils in IL-5-injected mice. The Inhibition of the above experimental eosinophilia wasalso observed by the passive administration of antl-IL-5 mAb. The results of the in vivo experiments clearly demonstrate that IL-5 plays an essential role In in vivo eoslnophllopoiesis and may be acting on eosinophils or their precursors directly through IL-5Rs, resulting in preferential growth of this lineage of hematopoletic cells. It can also be stressed that IL-5 regulates a specific lineage of hematopoietlc cells (eosinophils).
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