| Abstract
| - Transgenic mice (M54) containing a functional μ. heavy chain were examined to determine the effects of the transgeneon rearrangement and expression of endogenous immunoglobulin genes. Two major novel findings are presented. (I) In transgenic mice, the expressed endogenous VH repertoire In LPS-generated B cell blasts and hybrldomas is skewed towardexpression of JH-proxlmal VH families (VH7183 and Q52). (II) There is an increase in the frequency of B cells expressing λ light chain genes in transgenic mice. Furthermore, in Abelson-MuLV transformed pre-B cells, VH to DJH Is inhibited more than the D to JH rearrangement. The results presented indicate that the transgene skews the expressed VH repertoire by inhibiting the VH to DJH rearrangement while permitting an expansion of B cells expressing limited VH and λ light chain genes.
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