| Abstract
| - We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p- N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin Gtiters (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme- linked immunosorbent assay. rAls3p-N stimulated interferon g (IFN-γ) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-γ, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.
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