| Abstract
| - Background: Human T-cell leukemia virus type 1 (HTLV-1)can infect a number of cells of different lineages in vitro. yet the immunophenotypes of most aduly T-cell leukemia/lymphomas (ATLs) are restricted to CD4³. The apparent discrepancy between these findings is still largely unknown.Purpose: We report on a unique case of ATL in which the leukemia cells were positive for both T-cell and myeloid cell antigens. To characterize these cells, we isolasted cell lines from this patient with ATL. Methods: The fresh leukemia cells were cultured without the addition of interleukin-2. Cell cloning was carried out by limiting dilution. Results: A cell line (MU) and its clonasl sublines were established. Mu cells showed the same chromosomal abnormalities and T-cell receptor β-chain gene rearrangement pattern as those of fresh leukemia cells. MU cells were exclusively positive for a myeloid cell marker (CD13) but not for T-cell markers, despite the presence of T-cell receptor gene rearrangement. Conclusion: The established ATL cell line showed both T-cell and myeloid cell characteristics, which seems to be the first evidence for the close association of ATL cells with both lymphoid and myeloid features. The cell line may provide a new insight for the targets of HTLV-1 infection and transformation in vivo. [J Nastl Cancer Inst 84:690-693, 1992]
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