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Attributs	Valeurs
type	Concept
rdfs:label	METHODS Methods
skos:inScheme	http://hub.abes.fr/referentiel/ouparticlecategories
is Subject of	Measuring the gap: quantifying and comparing local health inequalities Labour participation of the chronically ill One pot general method for the derivatisation of polymer support for oligonucleotide synthesis Double-stranded DNA segments can efficiently prime the amplification of human genomic DNA An easy and fast alternative to plasmid shuffling for the identification of in vitro mutagenized alleles of essential genes of Saccharomyces cerevisiae Replication inhibition by nucleoside analogues of a recombinant Autographa californica multicapsid nuclear polyhedrosis virus harboring the herpes thymidine kinase gene driven by the IE-1(0) promoter: a new way to select recombinant baculoviruses A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer Phase Lock GelTM plasmid micropreps: direct insert screening, probe synthesis and sequencing within one day Cloning of RNA molecules in vitro High-performance liquid chromatographic determination of DNA base composition with fluorescence detection An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose An efficient method of generate phosphatase insensitive 3' labelled DNA probes using Taq polymerase cDNA selection from total yeast DNA containing YACs A rapid assay for quantitative detection of specific RNAs Solution hybridization with Hybond-mAP paper Simple method of RNA isolation from human leucocytic cell lines Single base pair mutation analysis by PNA directed PCR clamping Reduced occurrence of chimeric YACs in recombinationdeficient hosts Cloning biologically active geminivirus DNA using PCR and overlapping primers A simple method for isolation of intact RNA from dried polyacrylamide gels Direct sequencing of single primer PCR products: a rapid method to achieve short chromosomal walks Minisatellite isoalleles can be distinguished by single-stranded conformational polymorphism analysis in agarose gels Highly sensitive and specific amplification by primer digestion A high-throughput, non-radioactive microtiter plate assay for activity of the human immunodeficiency virus integrase protein Multivalent RNA probes and their use in the quantitation of multiple and non-homologous RNA species immobilized onto nylon membranes Single base substitutions are detected by double strand conformation analysis Partitioned pulsed-field gel electrophoresis-PCR (PPF-PCR): a new method for pulsed-field mapping for STS and microsatellites A new affinity purification procedure for DNA-binding proteins using bromoacetyl agarose Attenuation of PCR inhibition in the presence of plant compounds by addition of BLOTTO Random recombination of antibody single chain Fv sequences after fragmentation with DNasel in the presence of Mn2+ A rapid procedure for the compositional analysis of yeast artificial chromosomes 3′-5′ Proofreading-induced detection of point mutations by PCR using Tli DNA polymerase Screening differentially expressed cDNA clones obtained by differential display using amplified RNA Foresight in medicine A rapid method for the isolation of DNA-binding proteins from purified nuclei of tissues and cells in culture A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues Large-scale production of DNA sequencing templates by microtitre format PCR Sex determination using the hypomethylation of a human macro-satellite DXZ4 in female cells Microtiter format gene quantification by covalent capture of competitive PCR products: application to HIV-1 detection A simple mechanical procedure of produce encapsulated cells Addition of a Class IIS enzyme site in the mutagenic primer to improve two-step PCR-based targeted mutagenesis PCR priming from the restriction endonuclease site 3′extension Dipentafluorophenyl carbonate—a reagent for the synthesis of oligonucleotides and their conjugates A primer extension assay for modification of guanine by Ni(ll) complexes Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP Non-radioactive analysis of multiplexed microsatellite reactions using a direct blotting-sequencing appartatus Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry A highly sensitive method for mapping the 5′ termini of mRNAs Highly efficient DNA synthesis in isolated mitochondria from rat liver Specific detection of minus strand hepatitis A virus RNA by Tail-PCR following reverse transcription Efficient integration of artificial transposons into plasmid targets in vitro: a useful tool for DNA mapping, sequencing and genetic analysis Simplified in vivo subcloning for easy handling of large sets of lambda ZAP clones A versatile support for the synthesis of oligonucleotides of extended length and scale The mobility minima in pulsed-field capillary electrophoresis of large DNA Low concentrations of tetramethylammonium chloride increase yield and specificity of PCR Mutation detection using immobilized mismatch binding protein (MutS) A rapid detection of PCR amplification product using a new fluorescent intercalator; the pyrylium dye, P2 How do respondents understand survey questions on ill-health? Inter- and intra-observer variability in detection of patent foramen ovale with contrast echocardiography Cloning of Hepadnaviruses using microquantity serum Simplified DGGE in a horizontal electrophoresis system Expression analysis of multigene families by RT-PCR Oligonucleotide labeling methods 3. Direct labeling of oligonucleotides employing a novel, non-nucleosidic, 2-aminobutyl-1,3-propanediol backbone A means to reduce the complexity of oligonucleotides encoding degenerate peptides Use of oligonucleotides and nick translation for site-directed mutagenesis in plasmids In vivo UV-cross-linking hybridization: a powerful technique for isolating RNA binding proteins. Application to trypanosome mini-exon derived RNA Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization Analysis of oligonucleotide structure using hyperchromism measurements at long wavelengths A simple method of preparing plant samples for PCR Simulataneous Randomization of antibody CDRs by a synthetic ligase chain reaction strategy A method for the generation of YAC transgenic mice by pronuclear microinjection Yeast mitochondrial NAD+-dependent isocitrate dehydrogenase is an RNA-binding protein Exonuclease III induced ligase-free directional subcloning of PCR products Preparation of pure oligonucleotide-alkaline phosphatase conjugates A general and fast method for mapping mutations on the Escherichia coli chromosome ‘Race no more’: an alternative approach to cloning the 5′end of transcripts Ligation-independent cloning of PCR products with primers containing nonbase residues A new method for strand discrimination in sequence-directed mutagenesis Improved resolution of (CAC)n DNA fingerprints One step DNA sequencing of single-stranded DNA with reverse dye-primer A rapid scanning strip for tri- and dinucleotide short tandem repeats Low stringency-PCR (LS-PCR) allows entirely internally standardized DNA quantitation Efficient in situ electroporation of mammalian cells grown on microporous membranes Stability of oligodeoxynucleoside phosphorodithioates and phosphorothioates in aqueous ammonia Enhanced direct amplification of Guthrie card DNA following selective elution of PCR inhibitors cRACE: a simple method for identification of the 5′ end of mRNAs Evolving Methods in Genetic Epidemiology. III. Gene-Environment Interaction in Epidemiologic Research A new strategy useful rapid identification of microsatellites from DNA libraries with large size inserts Detection of single base differences using biotinylated nucleotides with very long linker arms An improved thermal cycle for two-step PCR-based targeted mutagenesis A solid phase method for mapping restriction sites Paml and Pamll restrictio endonucleases from Phormidium ambiguum PCR amplification of DNA sequences from nitrocellulose-bound, immunostained bacterial colonies Chopped inserts: a convenient alternative to agarose/DNA inserts or beads DNA extraction from Pleistocene bones by a silica-based purification method Sequencing of cDNA using anchored oligo dT primers

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