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type	Concept
rdfs:label	Methods Online Methods online
skos:inScheme	http://hub.abes.fr/referentiel/ouparticlecategories
is Subject of	Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications Improving specificity of DNA hybridization-based methods A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system One-step random mutagenesis by error-prone rolling circle amplification Negatively charged self-assembling DNA/poloxamine nanospheres for in vivo gene transfer RNA silencing in plants by the expression of siRNA duplexes Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells Proximity ligation assays with peptide conjugate ‘burrs’ for the sensitive detection of spores Distortion of quantitative genomic and expression hybridization by Cot-1 DNA: mitigation of this effect Iterative in vivo assembly of large and complex transgenes by combining the activities of φC31 integrase and Cre recombinase siRNA target site secondary structure predictions using local stable substructures Sequence dependence of cross-hybridization on short oligo microarrays A novel strategy for the identification of genomic islands by comparative analysis of the contents and contexts of tRNA sites in closely related bacteria Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes Cytoplasmic expression systems triggered by mRNA yield increased gene expression in post-mitotic neurons Multiplex sequencing of paired-end ditags (MS-PET): a strategy for the ultra-high-throughput analysis of transcriptomes and genomes Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line Accurate quantification of DNA methylation using combined bisulfite restriction analysis coupled with the Agilent 2100 Bioanalyzer platform Co-expression of anti-NFκB RNA aptamers and siRNAs leads to maximal suppression of NFκB activity in mammalian cells A large field CCD system for quantitative imaging of microarrays Hydroxyl radical footprinting in vivo: mapping macromolecular structures with synchrotron radiation Caenorhabditis elegans reporter fusion genes generated by seamless modification of large genomic DNA clones A 96-well DNase I footprinting screen for drug-DNA interactions Lentivirus-mediated antagomir expression for specific inhibition of miRNA function Free energy of DNA duplex formation on short oligonucleotide microarrays Dry-reagent disposable dipstick test for visual screening of seven leukemia-related chromosomal translocations An inducible system for expression and validation of the specificity of short hairpin RNA in mammalian cells Comparison of approaches for rational siRNA design leading to a new efficient and transparent method siRNA nanoformulation against the Ret/PTC1 junction oncogene is efficient in an in vivo model of papillary thyroid carcinoma Chemical mapping of cytosines enzymatically flipped out of the DNA helix Assessment of gene expression in many samples using vertical arrays Development of a single tube 640-plex genotyping method for detection of nucleic acid variations on microarrays Efficient delivery of RNA Interference to peripheral neurons in vivo using herpes simplex virus A protein-DNA docking benchmark The application of real-time PCR to the analysis of T cell repertoires Modeling genetic inheritance of copy number variations Microplate-based chromatin immunoprecipitation method, Matrix ChIP: a platform to study signaling of complex genomic events Predicting RNA-binding sites from the protein structure based on electrostatics, evolution and geometry Highly cost-efficient genome-wide association studies using DNA pools and dense SNP arrays Elevated α-synuclein mRNA levels in individual UV-laser-microdissected dopaminergic substantia nigra neurons in idiopathic Parkinson's disease wuHMM: a robust algorithm to detect DNA copy number variation using long oligonucleotide microarray data DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer Mutagenic inverted repeat assisted genome engineering (MIRAGE) In silico selection of RNA aptamers Zip Nucleic Acids: new high affinity oligonucleotides as potent primers for PCR and reverse transcription SubpathwayMiner: a software package for flexible identification of pathways A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli Alternative-splicing-based bicistronic vectors for ratio-controlled protein expression and application to recombinant antibody production HMMConverter 1.0: a toolbox for hidden Markov models Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization Accurate characterization of weak macromolecular interactions by titration of NMR residual dipolar couplings: application to the CD2AP SH3-C:ubiquitin complex Complete release of (5′S)-8,5′-cyclo-2′-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesions Structural analysis of hepatitis C RNA genome using DNA microarrays Effective and robust plasmid topology analysis and the subsequent characterization of the plasmid isoforms thereby observed Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference Genome-wide selection of unique and valid oligonucleotides A novel experimental approach for systematic identification of box H/ACA snoRNAs from eukaryotes A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells Suppression of vascular endothelial growth factor expression at the transcriptional and post-transcriptional levels Electrochemical detection of mismatched DNA using a MutS probe Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea Ubc9 fusion-directed SUMOylation identifies constitutive and inducible SUMOylation Positional artifacts in microarrays: experimental verification and construction of COP, an automated detection tool Site-directed transposon integration in human cells An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small molecules Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1 Rapid and efficient construction of markerless deletions in the Escherichia coli genome Consolidated strategy for the analysis of microarray spike-in data Fluorescence detection of single nucleotide polymorphisms using a universal molecular beacon Gene expression module-based chemical function similarity search Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs p53-dependent antiviral RNA-interference facilitates tumor-selective viral replication A genetic screen for components of the mammalian RNA interference pathway in Bloom-deficient mouse embryonic stem cells Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts In situ imaging and isolation of proteins using dsDNA oligonucleotides A simple, bead-based approach for multi-SNP molecular haplotyping Mapping the B-A conformational transition along plasmid DNA Real-time investigation of nucleic acids phosphorylation process using molecular beacons Methylation-Specific MLPA (MS-MLPA): simultaneous detection of CpG methylation and copy number changes of up to 40 sequences Transformation of isolated mammalian mitochondria by bacterial conjugation Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency Reproducibility, bioinformatic analysis and power of the SAGE method to evaluate changes in transcriptome Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ-32P]ATP in RNA structural investigation Genome physical mapping from large-insert clones by fingerprint analysis with capillary electrophoresis: a robust physical map of Penicillium chrysogenum Molecular beacons with a homo-DNA stem: improving target selectivity Primer Extension Enrichment Reaction (PEER): a new subtraction method for identification of genetic differences between biological specimens Sequence- and structural-selective nucleic acid binding revealed by the melting of mixtures Isolation of a site-specifically modified RNA from an unmodified transcript Diels-Alder cycloadditions in water for the straightforward preparation of peptide-oligonucleotide conjugates High-throughput trapping of secretory pathway genes in mouse embryonic stem cells mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis Sequence analysis and editing for bisulphite genomic sequencing projects Using force spectroscopy analysis to improve the properties of the hairpin probe An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans

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