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Attributs	Valeurs
type	Concept
rdfs:label	PROTOCOL Protocol
skos:inScheme	http://hub.abes.fr/referentiel/ouparticlecategories
is Subject of	Constructing a protein mutant database An automated approach for clustering an ensemble of NMR-derived protein structures into conformationally related subfamilies Efficient deletion mutagenesis by PCR Expression of soluble recombinant glycoproteins with predefined glycosylation: application to the crystallization of the T-cell glycoprotein CD2 Introduction of a histidine tail at the N-terminus of a secretory protein expressed in Escherichia coli A novel dialysis procedure for the crystallization of proteins A novel sequential procedure to enhance the renaturation of recombinant protein from Escherichia coil inclusion bodies A novel technique for containerless protein crystallization A simple and efficient synthesis of the inducer IPTG made for inexpensive heterologous protein production using the lac-promoter A strategy for optimizing charge reversal mutagenesis of ion pairs in hormone—receptor or enzyme—substrate complexes A highly specific and sensitive monoclonal antibody detecting histidine-tagged recombinant proteins Rapid and efficient site-specific mutagenesis pFab60: a new, efficient vector for expression of antibody Fab fragments displayed on phage The East Finland Berry and Vegetable Project: A health-related structural intervention programme Versatile protein engineering vectors for mutagenesis, expression and hybrid enzyme formation A rapid and efficient method for site-directed mutagenesis by PCR, using biotinylated universal primers and streptavidin-coated magnetic beads Recursive PCR: a novel technique for total gene synthesis A rapid method of calculating charge-charge interaction energies in proteins

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