This HTML5 document contains 15 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Subject Item

n2:w

rdf:type

rdac:C10001 bibo:Article

dcterms:isPartOf

n10:w

dcterms:subject

n13:technicalnote

dcterms:title

Dequenching of Cu(I)−Bathocuproinedisulfonate Complexes for High-Performance Liquid Chromatographic Determination of Phytochelatins, Heavy-Metal-Binding Peptides Produced by the Primitive Red Alga Cyanidioschyzon merolae

rdaw:P10072

n8:web n8:print

vivo:relatedBy

n12:3 n12:2 n12:1

marcrel:aut

n6:9b140a0b86527490a2f6bd37c1e6d8e3 n2:itokyoko n2:shirabetomoo

dcterms:abstract

A novel method has been devised for the determination of phytochelatins (PCs), heavy-metal-tolerant peptides produced by higher plants and algae. The method is based on the facts that fluorescence of bathocuproine disulfonate (BCS) is quenched by Cu(I) ions as a result of Cu(I)−BCS complex formation and that PCs compete with BCS for Cu(I). Detection of PCs via recovered fluorescence of BCS using the Cu(I)−BCS complex as a postcolumn reagent, following separation of peptides on an octyldecylsilane column, demonstrated a highly sensitive method for determination of PCs. PCs in the primitive red alga, Cyanidioschyzon merolae, grown in the presence or absence of added Cd(II) were successfully determined by this protocol. Unlike other methods for the determination of PCs, which rely on the SH groups in the peptides, the proposed method is unique in that detection is based on the chemical nature of PCs, which favors the formation of complexes with Cu(I). In this context, the new method yields chromatograms based on the strength of binding Cu(I) ions.

hub:isPartOfThisJournal

n4:w