This HTML5 document contains 33 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Subject Item

n2:w

rdf:type

bibo:Article rdac:C10001

dcterms:isPartOf

n10:w

dcterms:subject

n6:dmsodimethylsulphoxide n6:ph n6:5azadc5azadeoxycytidine n6:hifhypoxiainduciblefactor n6:invasion n6:irsimmunoreactivityscore n6:acidosis n6:gastriccancer n6:cmvcytomegalovirus n6:pcrpolymerasechainreaction n13:gastriccancer n14:pancreaticcancer n6:anhydrase n6:cacarbonicanhydrase

dcterms:title

Expression of carbonic anhydrase 9 at the invasion front of gastric cancers

rdaw:P10072

n4:web n4:print

vivo:relatedBy

n9:8 n9:9 n9:4 n9:1 n9:6 n9:3 n9:5 n9:7 n9:2

dcterms:abstract

Background: Carbonic anhydrase IX (MN/Ca9) catalyses the reversible metabolism of carbon dioxide to carbonic acid and has also been linked to malignant transformation and hypoxia in various cancers. Aims: To assess the expression and biological role of Ca9 in gastric cancer. Methods: Using gastric cancer cell lines and tissues, we studied expression of Ca9 by western blot analysis, immunohistochemistry, and polymerase chain reaction. Biological changes after Ca9 transfection and after treatment with 5′-azadeoxycytidine were also analysed in cancer cell lines. Results: Non-cancerous tissues strongly expressed Ca9 with membranous localisation. In contrast, Ca9 expression was frequently lost in gastric cancers (p<0.001). However, gastric cancers that retained Ca9 expression in cancer cells exhibited a shorter postoperative survival (p = 0.028). In vitro analysis revealed that loss of Ca9 expression in gastric cancer cell lines was restored after treatment with 5′-azadeoxycytidine and was associated with increased invasion (p<0.01). Moreover, AGS cells transfected with Ca9 exhibited significantly increased cell proliferation (p<0.05). Conclusions: A subgroup of gastric cancers retain Ca9 expression in cancer cells at the invasion front. While loss of Ca9 expression is regulated in part by methylation, re-expression of Ca9 is associated with increased invasion, supporting the hypothesis that increased Ca9 expression may contribute to invasion and thus advanced disease and tumour progression in a subset of gastric cancers.

hub:articleType

n15:other

hub:isPartOfThisJournal

n12:w

bibo:pmid

15951534