This HTML5 document contains 34 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Subject Item

n2:w

rdf:type

bibo:Article rdac:C10001

dcterms:isPartOf

n15:w

dcterms:subject

n8:estspresidentialaddress n8:30 n14:angiogenesis n14:stemcells n14:cytoprotection n14:transplantation n14:ischemia n8:31 n8:29

dcterms:title

Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function

rdaw:P10072

n6:web n6:print

vivo:relatedBy

n12:8 n12:7 n12:5 n12:6 n12:3 n12:4 n12:2 n12:1

marcrel:aut

n2:zhangping n7:748964e0a3e61c156e09750566a42395 n2:liuyonggang n2:zhangshaoheng n2:jiazhuqing n2:makangtao n2:linsongli n2:guojingxuan

dcterms:abstract

Objective: Heat shock proteins (HSPs) are cytoprotective proteins. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor. This study aimed to elucidate the possible role of cytoprotection and angiogenesis on cardiac function after bone marrow cell transplantation (BMT). Methods: Myocardial infarction was induced in inbred Lewis rats by left anterior descending artery ligation. A total of 5×106 bone marrow-mononuclear cells were transplanted into the ischemic zone by direct injection. At 1, 3, 7, 14 and 28 days post-transplantation, cardiac function was evaluated by echocardiography. The expressions of HSP32, HSP70 and VEGF were assessed by immunofluorescence and RT-PCR. The number of vessels was examined by immunohistochemistry. The differentiation of the transplanted cells was determined by immunofluorescence. Results: Echocardiography showed BMT led to sustained improvement in cardiac function, as assessed by left ventricle ejection fraction and fraction of shortening. Immunofluorescence revealed that the expressions of HSP32, HSP70 and VEGF were promoted in both transplanted bone marrow cells and recipient cardiomyocytes. RT-PCR showed that the mRNA expression levels of HSP32, HSP70 and VEGF in the BMT group were markedly higher in comparison with injection of peripheral blood cells or saline (P<0.01) by day 7. Seven days later, the vessel count showed that angiogenesis had been induced to a significantly greater degree in the BMT groups. Fourteen days later, specific markers for myocardial or vascular endothelial cells were detected in the transplanted bone marrow cells. Conclusions: BMT upregulated the expressions of HSP32, HSP70 and VEGF in both transplanted bone marrow cells and recipient endogenous cardiomyocytes in the early phase post-transplantation. This enhanced cytoprotection and angiogenesis, and contributed to the functional recovery following cardiac infarction. In the late phase, the transplanted bone marrow cells might differentiate into both myocardial and vascular endothelial cells that enhanced the ischemic cardiac function further.

hub:articleType

n10:researcharticle

hub:isPartOfThisJournal

n17:w