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n13:laserinducedfluorescence n13:confocalmicroscopy n13:dysplasia n13:autofluorescence n13:colonicmucosa
dcterms:title
Differences in laser-induced autofluorescence between adenomatous and hyperplastic polyps and normal colonic mucosa by confocal microscopy
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n2:kellyleo n2:fiarmangales n2:nathansonmichaelh n2:brianwesta n2:kapadiacyrusr n2:deckelbaumlawrencei
dcterms:abstract
Abstract. Laser-induced autofluorescence has been used to discriminate normal from adenomatous colonic mucosa. However, few studies to date have studied the origin of colonic autofluorescence. Using confocal microscopy (excitation wavelength 488 nm), we have shown that autofluorescence at this wavelength is present predominantly in the lamina propria of normal mucosa but in the epithelium in adenomatous and hyperplastic polyps. The intensity ratio of epithelial cell to lamina propria fluorescence was significantly lower (P<0.0001) in normal mucosa (0.52±0.01) compared with either adenomatous (1.6±0.2) or hyperplastic polyps (1.7±0.15). However, the ratios were not significantly different between hyperplastic and adenomatous polyps. Thus, confocal microscopy enables the detection of the sites of autofluorescence within colonic mucosa and the quantitation of differences in fluorescence between different tissue types.
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n10:originalpaper n16:gastrointestinaloncology
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BF02065535
dcterms:dateCopyrighted
1995
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