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n11:mucin n11:ulcerativecolitis n11:wholegutlavagefluid n11:crohnsdisease
dcterms:title
Characterization of mucin in whole-gut lavage fluid obtained from patients with inflammatory bowel disease
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n2:nakamuratoshiya n2:saitohhiromi n2:yoshidayutaka n2:endomasahiko n2:takagakikeiichi n2:munakataakihiro
dcterms:abstract
Abstract. Whole-gut lavage fluid, collected by administering an electrolyte lavage solution orally, was found to be an excellent and easily collectable source of abundant mucin. Furthermore, the biochemical features of the mucin from patients with ulcerative colitis and Crohn's disease were investigated. The mucin was separated into four fractions by Sepharose CL-4B, Sepharose CL-2B, and DEAE Sephacel chromatography. Compared with healthy subjects, the total yields of mucin from ulcerative colitis patients were low due to a deficiency of neutral mucin, whereas those from Crohn's disease patients were high, which was attributable mainly to high-molecular-weight mucin. The fucose and sulfate contents were low in ulcerative colitis, but only the former was low in Crohn's disease. The different biochemical features of the mucin obtained from whole gut lavage fluid appear to reflect mucosal pathological changes associated with inflammatory bowel disease.
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n7:intestinaldisordersinflammatoryboweldiseaseimmunologyandmicrobiology n14:originalpaper
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BF02088743
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1996
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