. "mbcb100034e" . . "\u00A9 SFMBCB 2010" . "An error has been introduced during the publication process of the original article (Figure 5, 6 and 7 are missing). The evaluation of innovative bone substitutes requires the development of an optimal model close to physiological conditions. An interesting alternative is the use of an immortalized cell line to construct multicellular spheroids, that is, three-dimensional (3D) cultures. In this study, a modified hanging drops method has resulted in the generation of spheroids with a well established human Fetal OsteoBlasts line (hFOB 1.19), and tests have been focused on the effect of 45S5 bioglass ionic dissolution products in comparison with two-dimensional (2D) cultures. Depending on cell culture type, quantitative analysis (cell proliferation, viability/cytotoxicity, cellular cycle) and qualitative analysis (electron microscopy, genes expression) showed a differential effect. Cell proliferation was enhanced in 2D conditioned cultures in accordance with literature data, but decreased in 3D cultures submitted to the same conditions, without change of gene expression patterns. The decrease of cell proliferation, observed in conditioned spheroids, appears to be in agreement with clinical observations showing the insufficiency of commercially available bioglasses for bone repairing within non bearing sites, such as periodontal defects or small bone filling in general. Therefore, we suggest that this model could be adapted to the screening of innovative bioactive materials by laboratory techniques already available and extended monitoring of their bioactivity. Also, it could be applied to the co-cultivation of mixed cell populations, for example, co-culture of endothelial and bone cells that would be a model of interest for the study of biomaterials implanted in bone site. Furthermore, it might be of interest to study the effect of biomaterials on stem cells or osteoblasts harvested from the patient and cultivated in 3D spheroids as a scaffold in tissue engineering applications."@en . . "Mise au point d\u2019un mod\u00E8le tridimensionnel pour l\u2019\u00E9valuation des biosubstituts osseux in vitro"@fr . . . . . . . . . . "SFMBCB" . . "2010"^^ . . . . . . . . . . . "2010"^^ . . . "Durant le processus de publication, une erreur est apparue dans l\u2019article original (les Figures 5, 6 et 7 n\u2019ont pas \u00E9t\u00E9 publi\u00E9es). L\u2019\u00E9valuation des substituts osseux n\u00E9cessite la mise au point d\u2019un mod\u00E8le de culture le plus proche possible du mod\u00E8le physiologique. Une alternative int\u00E9ressante consiste en l\u2019utilisation d\u2019une lign\u00E9e osseuse immortalis\u00E9e pour la pr\u00E9paration de sph\u00E9ro\u00EFdes multicellulaires correspondant \u00E0 une culture tridimensionnelle (3D). Dans ce travail, l\u2019adaptation d\u2019une technique en gouttes suspendues a abouti \u00E0 la g\u00E9n\u00E9ration de sph\u00E9ro\u00EFdes \u00E0 partir de la lign\u00E9e standardis\u00E9e hFOB1.19 (human Fetal OsteoBlast) et les tests ont port\u00E9 sur l\u2019\u00E9tude de l\u2019effet des produits ioniques de dissolution du bioverre 45S5 en comparaison avec des cultures bidimensionnelles (2D). Selon le type de culture, les analyses quantitatives (prolif\u00E9ration cellulaire, viabilit\u00E9/cytotoxicit\u00E9, cycles cellulaires) et qualitatives (microscopie \u00E9lectronique, expression des marqueurs g\u00E9n\u00E9tiques) ont montr\u00E9 un effet diff\u00E9rentiel du bioverre. En effet, la prolif\u00E9ration cellulaire est augment\u00E9e en cultures 2D conditionn\u00E9es conform\u00E9ment aux donn\u00E9es de la litt\u00E9rature mais, si l\u2019on conserve le m\u00EAme conditionnement, elle est significativement diminu\u00E9e en cultures 3D sans modification de l\u2019expression g\u00E9nique dans les deux cas. Ce ralentissement de la prolif\u00E9ration cellulaire, constat\u00E9e au sein des sph\u00E9ro\u00EFdes, para\u00EEt \u00EAtre en accord avec l\u2019inefficacit\u00E9 clinique des bioverres commercialement disponibles pour la r\u00E9paration osseuse en sites non porteurs tels que les d\u00E9fauts parodontaux et les comblements de d\u00E9fauts critiques en g\u00E9n\u00E9ral. D\u2019o\u00F9 l\u2019int\u00E9r\u00EAt du mod\u00E8le sph\u00E9ro\u00EFde qui pourrait \u00EAtre adapt\u00E9 \u00E0 l\u2019\u00E9valuation rapide de biomat\u00E9riaux innovants, par des techniques de laboratoire couramment utilis\u00E9es, et \u00E0 l\u2019\u00E9tude de leurs effets bioactifs sur une p\u00E9riode prolong\u00E9e."@fr . . . . .